Invitrogen™ Human Hepsin ELISA Kit

Catalog No.	Quantity
EH240RBX10	10 x 96 Tests

Catalog No. EH240RBX10 Supplier Invitrogen™ Supplier No. EH240RBX10

Description

ELISA

Human Hepsin quantitates human Hepsin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Hepsin.

Hepsin, encoded by the HPN gene, is a type II transmembrane serine protease prominently expressed in tissues such as the liver, kidney, and prostate. Hepsin plays a crucial role in proteolytic processes involved in extracellular matrix remodeling, cell growth, and development. It is known for activating precursor molecules, including hepatocyte growth factor (HGF), which is essential for liver development and regeneration. Furthermore, hepsin's involvement extends to influencing cell signaling pathways related to tissue homeostasis and repair. In the context of cancer, particularly prostate cancer, hepsin is implicated in tumor progression and metastasis by promoting extracellular matrix degradation, thereby facilitating tumor invasion and spread. Aberrant expression or activity of hepsin is associated with pathophysiological changes in the tumor microenvironment, making it a target for therapeutic interventions aimed at controlling cancer progression. Research continues to explore hepsin's biochemical functions and regulatory mechanisms, providing insights into its potential roles in both normal physiology and disease states.

Specifications

• Accession Number: P05981
• Assay Range: 61.5-15000 pg/mL
• Assay Sensitivity: 61.5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: hepsin; hepsin (transmembrane protease, serine 1); Hlb320; HPN; Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non-catalytic chain; testicular tissue protein Li 85; TMPRSS1; Transmembrane protease serine 1hepsin; hepsin (transmembrane protease, serine 1); Hlb320; HPN; Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non-catalytic chain; testicular tissue protein Li 85; TMPRSS1; Transmembrane protease serine 1;
• Gene ID (Entrez): 3249
• Gene Symbol: HPN
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Hepsin
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min