Invitrogen™ Human ICAM-1 (CD54) Fc Chimera Recombinant Protein

Catalog No.	Quantity
PIRP88056	100 μg

Catalog No. PIRP88056 Supplier Invitrogen™ Supplier No. RP88056

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (800 μL for 1 mg and 80 μL for 100 μg) be added to the vial to prepare a stock solution of 1.25 mg/mL. Concentration is measured by UV.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Specifications

• Accession Number: P05362
• Concentration: 0.25 mg/mL
• For Use With (Application): Control, Neutralization
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 3383
• Molecular Weight (g/mol): 76.2 kDa
• Name: Human ICAM-1 (CD54) Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells
• Common Name: ICAM-1 (CD54)
• Gene Symbol: ICAM1
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human ICAM1, amino acids Met1-Glu480 (Accession # NP_000192.2) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC