Invitrogen™ Human ICAM-1 (CD54) His-tag Fc Chimera Recombinant Protein

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Specifications

• Accession Number: P05362
• For Use With (Application): ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 3383
• Molecular Weight (g/mol): 77.4 kDa
• Name: Human ICAM-1 (CD54) His-tag Fc Chimera
• Quantity: 5 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Gene Alias: BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells
• Common Name: ICAM-1 (CD54)
• Gene Symbol: ICAM1
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >40% cells will adhere after 1 hour of incubation (at 37°C.)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag, His-tag
• Sequence: Human ICAM1, amino acids Met1-Glu480 (Accession # NP_000192.2) with a C-terminal His-tagged human IgG1 Fc-tag
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% by SDS-PAGE