Gibco™ Human ICAM-3 Fc Recombinant Protein, PeproTech®

Catalog No.	Quantity
150-23-50UG	50 μg
15023100UG	100 μg
150-23-10UG	10 μg
150-23-1MG	1 mg
15023250UG	250 μg
15023500UG	500 μg

Catalog No. 150-23-50UG Supplier Gibco™ Supplier No. 1502350UG

Description

Recombinant Protein

PeproTech's CHO-derived Recombinant Human ICAM-3 Fc is a glycosylated, disulfide-linked homodimer of 689 amino-acid-residues whose monomer consists of the 456-amino-acid extracellular portion of ICAM-3 fused to the 231-amino-acid length Fc portion of human IgG by two glycine residues. The calculated molecular weight of monomeric CHO cell-derived Recombinant Human ICAM-3 Fc is 75.5 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 130-140 kDa by SDS-PAGE analysis under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

Specifications

• Accession Number: P32942
• For Use With (Application): Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 3385
• Molecular Weight (g/mol): 75.5 kDa
• Name: Human ICAM-3 Fc
• Purification Method: Purified
• Quantity: 50 μg
• Source: CHO cells
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: CD50; CDw50; ICAM3; ICAM-3; ICAM-R; Intercellular adhesion molecule 3; intercellular adhesion molecule-3
• Common Name: CD50 (ICAM3)
• Gene Symbol: ICAM3
• Biological Activity: ICAM-3 Fc supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated with 1.0-4.0 ug/ml ICAM-3 Fc concentration
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: QEFLLRVEPQ NPVLSAGGSL FVNCSTDCPS SEKIALETSL SKELVASGMG WAAFNLSNVT GNSRILCSVY CNGSQITGSS NITVYRLPER VELAPLPPWQ PVGQNFTLRC QVEDGSPRTS LTVVLLRWEE ELSRQPAVEE PAEVTATVLA SRDDHGAPFS CRTELDMQPQ GLGLFVNTSA PRQLRTFVLP VTPPRLVAPR FLEVETSWPV DCTLDGLFPA SEAQVYLALG DQMLNATVMN HGDTLTATAT ATARADQEGA REIVCNVTLG GERREARENL TVFSFLGPIV NLSEPTAHEG STVTVSCMAG ARVQVTLDGV PAAAPGQPAQ LQLNATESDD GRSFFCSATL EVDGEFLHRN SSVQLRVLYG PKIDRATCPQ HLKWKDKTRH VLQCQARGNP YPELRCLKEG SSREVPVGIP FFVNVTHNGT YQCQASSSRG KYTLVVVMDI EAGSSHGGPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
• Content And Storage: -20°C
• Activity: ICAM-3 Fc supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated with 1.0-4.0 ug/ml ICAM-3 Fc concentration
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.