Invitrogen™ Human IFN-alpha A (alpha 2a) (Carrier-Free) Recombinant Protein

Catalog No.	Quantity
PI111012	20 μg

Catalog No. PI111012 Supplier Invitrogen™ Supplier No. 111012

Description

Recombinant Protein

After receipt, the product may be stored at -20°C for short-term use (less than 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.

Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.

Specifications

• Accession Number: P01563
• Concentration: 0.2 mg/mL
• For Use With (Application): ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3440
• Molecular Weight (g/mol): 19.2 kDa
• Name: Human IFN-alpha A (alpha 2 A) (Carrier-Free)
• Quantity: 20 μg
• Source: E. Coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: alpha-2 A interferon; IFNA; IFNA2; IFNA2A; IFNA2B; IFNA2C; IFN-alpha-2; IFN-alphaA; INFA2; Interferon; interferon alpha 2; interferon alpha 2 A; interferon alpha 2 b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; leIF A
• Common Name: Interferon alpha-2 A
• Gene Symbol: IFNA2
• Biological Activity: EC50 ̃ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758 ]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394 ]. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-alpha-A) provided by the National Institutes of Health [see Pestka, S. (1986). Methods in Enzymology, Academic Press, New York 119, 14-23 ]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV).
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%