Invitrogen™ Human IFN-alpha A (alpha 2a) Recombinant Protein

Description

Recombinant Protein

Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.

Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.

Specifications

• Accession Number: P01563
• Concentration: Lot-specific
• For Use With (Application): ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with 0.1% BSA and no preservative
• Gene ID (Entrez): 3440
• Molecular Weight (g/mol): 19.2 kDa
• Name: Human IFN-alpha A (alpha 2 A)
• Quantity: >5 x 10^6 units
• Source: E. Coli
• Regulatory Status: RUO
• Gene Alias: alpha-2 A interferon; IFNA; IFNA2; IFNA2A; IFNA2B; IFNA2C; IFN-alpha-2; IFN-alphaA; INFA2; Interferon; interferon alpha 2; interferon alpha 2 A; interferon alpha 2 b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; leIF A
• Common Name: Interferon alpha-2 A
• Gene Symbol: IFNA2
• Biological Activity: EC50 ̃ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Budd et al. (1985) Canc. Chem. Pharm. 12:39 ]. The units are determined by use of a Human IFN Alpha A (Hu IFN- alpha A [2 a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A provided by the National Institutes of Health [Meager et al. (2001) J. Immunol Methods. 257(1-2):17-33 ].
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant