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Invitrogen™ eBioscience™ Human IFN gamma ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement of human IFN gamma protein levels from samples including serum, plasma, and cell culture supernatants.

Manufacturer:  Invitrogen™ 88731686

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Catalog No. 50-173-27


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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Description

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
Specifications

Specifications

P01579
Biotin
3458
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Human
1 hr 45 min
Human
Interferon-gamma, IFN-g, IFNg
4-500 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
10 x 96 tests
4 pg/mL
Plasma (100μL), Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
IFNG
HRP
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