Gibco™ Human IFN-gamma Recombinant Protein

Catalog No.	Quantity
ENRIFNG100	100 μg

Catalog No. ENRIFNG100 Supplier Gibco™ Supplier No. RIFNG100

Description

Recombinant Protein

For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Reconstitute with 0.5 mL of distilled water.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01579
• For Use With (Application): Bioactivity, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3458
• Molecular Weight (g/mol): 16.7 kDa
• Name: Human IFN-gamma
• Purification Method: Purified
• Quantity: 100 μg
• Source: E. Coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
• Common Name: IFN gamma
• Gene Symbol: IFNG
• Biological Activity: ED50 ≤ 0.05 ng/mL; determined by a dose-dependent cytotoxicity assay using HT-29 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: ED50 ≤ 0.05 ng/mL; determined by a dose-dependent cytotoxicity assay using HT-29 cells.
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant