Invitrogen™ Human IgE ELISA Kit

Catalog No.	Quantity
50-112-5320	96 Tests
50-112-5322	10 x 96 Tests

Catalog No. 50-112-5320 Supplier Invitrogen™ Supplier No. BMS2097

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IgE

ELISA

The Human Immunoglobulin E (Hu IgE) ELISA quantitates Hu IgE in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgE. Principle of the method The Human IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IgE is one of the five classes of immunoglobulins found in mammals, characterized by its monomeric structure consisting of two heavy (epsilon) chains with four Ig-like constant domains and two light chains. Although IgE comprises only a small portion of the total immunoglobulin in serum, it plays a crucial role in type I hypersensitivity reactions, such as hay fever, asthma, hives, and anaphylactic shock. IgE is produced by terminally differentiated plasma B cells and basophils. It binds to Fc epsilon receptors on mast cells and basophils, leading to the release of histamine and other molecules that promote allergic responses. Crosslinking of IgE bound to these receptors induces degranulation of mast cells and basophils, resulting in allergic manifestations. In addition to its role in allergic responses, IgE is functionally important for immunity against parasites, highlighting its dual role in both protective and pathological immune processes.

Specifications

• Accession Number: P01854
• Assay Range: 7.8-500 ng/mL
• Assay Sensitivity: 0.5 ng/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human IgE
  HRP-Conjugate anti-human IgE monoclonal antibody
  human total IgG Standard lyophilized, 1000 ng/mL upon reconstitution
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Blue-Dye
  Green-Dye
  Adhesive Films
  
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: HCAK1; IgA1; IgG3; IGHA1; IGHA2; IGHG1; IGHG2; IGHG3; IGHG4; IGKC; IGKCD; IGLC; IGLC1; Immunoglobulin E; immunoglobulin heavy constant alpha 1; immunoglobulin heavy constant alpha 2 (A2m marker); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); immunoglobulin kappa constant; immunoglobulin lambda constant 1; ImmunoglobulinE; KmHCAK1; IgA1; IgG3; IGHA1; IGHA2; IGHG1; IGHG2; IGHG3; IGHG4; IGKC; IGKCD; IGLC; IGLC1; Immunoglobulin E; immunoglobulin heavy constant alpha 1; immunoglobulin heavy constant alpha 2 (A2m marker); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); immunoglobulin kappa constant; immunoglobulin lambda constant 1; ImmunoglobulinE; Km;
• Gene ID (Entrez): 3497
• Gene Symbol: IGHE
• Interassay CV: 5.2%
• Intraassay CV: 6.6%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer Concentrate, TMB Substrate Solution, Stop Solution, Controls, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL
• Storage Requirements: 2-8°C
• Synonym: Immunoglobulin E
• Target: Human IgE
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 30 min