Invitrogen™ Human IgG1 Fc (103 Cys/Ser) Recombinant Protein

Catalog No.	Quantity
PIRP88063	100 μg
PIRP88064	1 mg

Catalog No. PIRP88063 Supplier Invitrogen™ Supplier No. RP88063

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. Reconstituted with sterile deionized water to 0.25 mg/mL. Concentration is measured by UV.

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). [UniProt].

Specifications

• Accession Number: P01857
• Concentration: 0.25 mg/mL
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 3500
• Molecular Weight (g/mol): 26.08 kDa
• Name: Human IgG1 Fc (103 Cys/Ser)
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: IgG1; IgG3; Igh-4; Ighg; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin heavy chain (gamma polypeptide); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); RGD1359539; VH7183
• Common Name: IGHG1
• Gene Symbol: IGHG1
• Biological Activity: 1. Measured by its ability to bind human CD16a-His in a functional ELISA. 2. Measured by its ability to bind human CD16a-AVI-His in a functional ELISA. 3.Measured by its ability to bind human CD16a-His in a functional ELISA.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Human IgG1 Fc, amino acids Glu99-Lys330 (Accession # P01857-1) with a 103 Cys/Ser mutation
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized Recombinant Human IgG1-Fc Protein (103 Cys/Ser) (Cat: 10702-HNAH) at 2μg/mL (100μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His Tag) (Cat: 10256-H08H), the EC50 is 1.6-4.8 ng/mL
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.