Invitrogen™ Human IgG3 ELISA Kit

Catalog No.	Quantity
50-112-5308	96 Tests

Catalog No. 50-112-5308 Supplier Invitrogen™ Supplier No. BMS2094

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IgG3

ELISA

The Human Immunoglobulin G3 (Hu IgG3) ELISA quantitates Hu IgG3 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG3. Principle of the method The Human IgG3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgG3, also known as human immunoglobulin G3, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the least abundant subclass of IgG in the blood and plays a minor role in defense against pathogens. IgG3 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG3 is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG3 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.

Specifications

• Assay Range: 4.7-300 ng/mL
• Assay Sensitivity: 0.21 ng/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human IgG3
  HRP-Conjugate anti-human IgG monoclonal antibody
  human IgG3 Standard lyophilized, 600 ng/mL upon reconstitution
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Blue-Dye
  Green-Dye
  Adhesive Films
  
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: IgG3; IGHG3; immunoglobulin heavy constant gamma 3 (G3m marker)IgG3; IGHG3; immunoglobulin heavy constant gamma 3 (G3m marker);
• Interassay CV: 3%
• Intraassay CV: 5.6%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL
• Storage Requirements: 2-8°C
• Synonym: Immunoglobulin G2
• Target: Human IgG3
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 30 min