Gibco™ Human IL-1 alpha Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized human IL-1 alpha should be reconstituted in deionized water to 0.1-1.0 mg/mL to regain full activity. These stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in low endotoxin medium or buffered solution with heat inactivated FCS or tissue culture grade BSA. Storage: Lyophilized human IL-1 alpha should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human IL-1 alpha at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Specifications

• Accession Number: P01583
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 3552
• Molecular Weight (g/mol): 18 kDa
• Name: Human IL-1 alpha
• Purification Method: Membrane-Filtered
• Quantity: 100 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: CTLA8; hematopoietin-1; IL 1 A; IL 1 A; IL1; IL-1 alpha; IL17; IL1A; Il-1 A; IL-1 alpha; IL1-ALPHA; IL1F1; IL1a; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1 A; interleukin 1-alpha; interleukin 1-alpha (AA 1 - 152); Interleukin1 alpha; interleukin-1 alpha; interleukin-1 alpha precursor; Interleukin-17 A; preinterleukin 1 alpha; pre-interleukin-1 alpha; pro-interleukin-1-alpha; RP23-160G19.8
• Common Name: IL-1 alpha
• Biological Activity: ED50 = 0.001 - 0.005 ng/mL; determined by the dose-dependent proliferation of mouse D10S cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
• Content And Storage: 4°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Cell Cycle & Proliferation, Immunology, Angiogenesis
• Product Line: Gibco™
• Purity or Quality Grade: >95% by SDS-PAGE