Invitrogen™ Human IL-1 beta Recombinant Protein

Catalog No.	Quantity
A42509	50 μg
A42508	5 μg

Catalog No. A42509 Supplier Invitrogen™ Supplier No. A42509

Description

Recombinant Protein

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Accession Number: P01584
• For Use With (Application): Control, Bioactivity, Immunohistochemistry, Western Blot
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 3553
• Molecular Weight (g/mol): 17 kDa
• Name: Human IL-1 beta
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Source: E. coli
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Gene Alias: catabolin; cytokine; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1; IL1 B; IL-1 beta; IL-1 precursor; IL1B; IL-1B; IL1B1; IL1beta; IL-1beta; IL1-BETA; IL1F2; IL1β; ILN; Interleukin; interleukin 1 beta; Interleukin 1 beta precursor; interleukin 1, beta; interleukin 1, beta 1; interleukin 1-beta; interleukin*1*beta; Interleukin1 beta; interleukin-1 beta; interleukin-1 beta precursor; interleukin-1 beta precursor (AA -113 to 153); interleukin-1 beta proprotein; interleukin-1b; interleukin-1beta; interleukin-beta; LAF; lymphocyte proliferation-potentiating factor; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; prointerleukin-1 beta; pro-interleukin-1-beta
• Common Name: IL-1 beta
• Gene Symbol: IL1B
• Biological Activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.2-2 ng/mL
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: Untagged
• Sequence: Human IL-1beta, amino acids Ala117-Ser269 (Accession # NP_000567.1)
• Content And Storage: Includes 1 vials of protein in suspension buffer. Store at -20 °C.
• Activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.2-2 ng/mL.
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Form: Lyophilized
• Research Category: Cell Cycle & Proliferation, Immunology, Oncology
• Purity or Quality Grade: ≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
• Protein: interleukin 1, beta