Invitrogen™ Human IL-1 F6 ELISA Kit

Description

ELISA

Human IL-1 F6 quantitates human IL-1 F6 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-1 F6.

The IL-1 family is comprised of 11 structurally related ligands, including recently re-named IL-36alpha (IL-1F6), beta (IL-1F8), and gamma (IL-1F9). IL-36alpha is highly expressed in psoriatic plaques, as are IL-36beta and IL-36gamma, and is the only IL-1 family member found to be expressed by T cells, as well as monocytes and B cells. Primarily found in skin and lymphoid tissues, IL-36alpha is also found to a lesser extent in tissues of the fetal brain, trachea, stomach, and intestine. IL-36alpha signals through a combination of the IL-1Rrp2 (IL-1R6) receptor, which is primarily expressed on certain dendritic cells, and the widely expressed IL-RAcP (IL-1 receptor accessory protein). IL-36alpha also functions as an agonist of NF-kappaB and MAPK pathways, and induces the production of proinflammatory cytokines and chemokines, including IL-6, IL-8, BD-2, and BD-3.

Specifications

• Accession Number: Fil1; FIL1 epsilon; FIL1(EPSILON); Fil1e; Fil1epsilon; IL-1 epsilon; IL1(EPSILON); IL1E; IL1F6; IL-1F6; IL-1F6 (FIL-1-epsilon); Il1f9; Il1h1; IL-1H1; IL1RP2; Il36a; interleukin 1; interleukin 1 family, member 6; interleukin 1 family, member 6 (epsilon); interleukin 1 family, member 9; interleukin 1, epsilon; interleukin 36, alpha; interleukin-1 epsilon; Interleukin-1 family member 6; interleukin-1 homolog 1; Interleukin-36; Interleukin-36 alpha
• Assay Range: 0.8 to 200 ng/mL
• Assay Sensitivity: 0.8 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 27179
• Gene Symbol: IL36A
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IL1F6
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins