Invitrogen™ Human IL-18 ProQuantum Immunoassay Kit

Catalog No.	Quantity
A35613	96 Tests
A356135	5 x 96 Tests

Catalog No. A35613 Supplier Invitrogen™ Supplier No. A35613

Description

ELISA

The ProQuantum Human IL-18 Immunoassay Kit is designed to provide quantitative measurements of human IL-18 in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours. High sensitivity-detect low levels of protein with greater sensitivity than traditional methods Broad dynamic range-?5 logarithmic units, minimizing sample dilutions to ensure they fall within the range Small sample consumption-use 2-5 μL of sample (compared to 75 μL for triplicate wells with other methods) Fast, easy workflow-2 hours from sample to answer, with no wash steps No proprietary instrument to purchase-runs on any real-time PCR instrument ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1). The assay work...

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Specifications

• Accession Number: Q14116
• Assay Range: 0.064-1000 pg/mL
• Assay Sensitivity: <0.05 pg/mL
• Conjugate: FAM
• Immunoassay Kit Format: Proximity-based Immunoassay
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): qPCR Instruments
• Gene Alias: cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320;
• Gene ID (Entrez): 3606
• Gene Symbol: IL18
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Shipping Condition: Dry Ice
• Storage Requirements: -20°C
• Target: IL-18
• Test Time: 30 min
• Total Assay Time: 2 hr