Invitrogen™ Human IL-27 ELISA Kit

Catalog No.	Quantity
EH271RBX10	10 x 96 Tests

Catalog No. EH271RBX10 Supplier Invitrogen™ Supplier No. EH271RBX10

Description

ELISA

Human IL-27 quantitates human IL-27 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-27.

IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.

Specifications

• Accession Number: Q14213, Q8NEV9
• Assay Range: 81-20000 pg/mL
• Assay Sensitivity: 81 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus induced gene 3 (similar to Interleukin-27 beta chain precursor); epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; Il27; IL-27; IL27 p28; IL-27 p28 subunit; IL27 subunit; IL-27 subunit alpha; IL-27 subunit beta; Il27a; IL-27A; IL-27-A; IL27-A; Il27b; IL-27B; IL27p28; IL-27p28; Il30; IL35 subunit; ILN; Interleukin; interleukin 27; interleukin 30; interleukin-27 subunit alpha; Interleukin-27 subunit beta; interleukin-30; MGC71873; M-IL-27 (p28); p28; RGD1561420cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus induced gene 3 (similar to Interleukin-27 beta chain precursor); epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; Il27; IL-27; IL27 p28; IL-27 p28 subunit; IL27 subunit; IL-27 subunit alpha; IL-27 subunit beta; Il27a; IL-27A; IL-27-A; IL27-A; Il27b; IL-27B; IL27p28; IL-27p28; Il30; IL35 subunit; ILN; Interleukin; interleukin 27; interleukin 30; interleukin-27 subunit alpha; Interleukin-27 subunit beta; interleukin-30; MGC71873; M-IL-27 (p28); p28; RGD1561420;
• Gene ID (Entrez): 10148, 246778
• Gene Symbol: Ebi3, IL27
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL-27
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min