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  5. Invitrogen™ Human R-Spondin 2 ELISA Kit 

Invitrogen™ Human R-Spondin 2 ELISA Kit

Catalog No. EH396RBX5
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Description

ELISA

Human R-Spondin 2 quantitates human R-Spondin 2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human R-Spondin 2.

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally-related, secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. This activity effectively activates and increases the subsequent nuclear signaling of beta-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/beta-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 2610028F08Rik; AA673245; CRISTIN2; Cristin-2; cysteine-rich and single thrombospondin domain-containing protein 2; D430027K22; ftls; hRspo2; mCristin-2; RGD1562331; roof plate-specific spondin-2; Rspo2; R-spondin 2; R-spondin 2 homolog; R-spondin 2 homolog (Xenopus laevis); R-spondin-2; UNQ9384/PRO34209
Assay Range 0.55 to 35 ng/mL
Assay Sensitivity 0.55 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 340419
Gene Symbol RSPO2
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target RSPO2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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