Gibco™ Human IL-28A (IFN-lambda 2) Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human IFN-lambda2 is a 19.6 kDa protein containing 174 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role

Specifications

• Accession Number: Q8IZJ0
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 282616
• Molecular Weight (g/mol): 19.6 kDa
• Name: Human IL-28 A (IFN-lambda 2)
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: cytokine Zcyto20; EG330496; Ifnl2; IFN-lambda2; IFN-lambda-2; Il28a; IL-28 A; ILN; interferon lambda 2; interferon lambda-2; interferon, lambda 2; interferon-lambda 2; interferon-lambda2; Interleukin; interleukin 28 A; interleukin 28 A (interferon, lambda 2); interleukin-28 A; ZCYTO20
• Common Name: IL-28 A
• Gene Symbol: IFNL2
• Biological Activity: Determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells. The expected ED50 is 0.3-0.5 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: PVARLHGALP DARGCHIAQF KSLSPQELQA FKRAKDALEE SLLLKDCRCH SRLFPRTWDL RQLQVRERPM ALEAELALTL KVLEATADTD PALVDVLDQP LHTLHHILSQ FRACIQPQPT AGPRTRGRLH HWLYRLQEAP KKESPGCLEA SVTFNLFRLL TRDLNCVASG DLCV
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.