Gibco™ Human IL-3 Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human IL-3 in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA. Storage: Lyophilized recombinant human IL-3 should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted recombinant human IL-3 at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Specifications

• Accession Number: P08700
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 3562
• Molecular Weight (g/mol): 15 kDa
• Name: Human IL-3
• Purification Method: Sequential Chromatography
• Quantity: 50 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF
• Common Name: IL-3
• Biological Activity: ED50 = 0.1 - 0.25 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: APMTQTTPLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF
• Content And Storage: 4°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Stem Cell Research, Immunology
• Product Line: Gibco™
• Purity or Quality Grade: >95% by SDS-PAGE

Safety and Handling

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