Gibco™ Human IL-31 Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, dilute the constituted protein in buffered solution containing carrier protein, such as PBS with 0.1% BSA. Apportion the diluted protein into working aliquots, and store at ≤-20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C to -80°C for long-term storage, preferably desiccated. The reconstituted protein is stable at 2-8°C for up to 1 week. Working aliquots stored with a carrier protein are stable for at least 3 months at ≤-20°C to ≤-80°C. Avoid repeated freeze-thaw cycles.

Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.

Specifications

• Accession Number: Q6EBC2
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 10 mM sodium phosphate with no preservative; pH 7.5
• Gene ID (Entrez): 386653
• Molecular Weight (g/mol): 15.8 kDa
• Name: Human IL-31
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: 1700013B14Rik; IL31; IL-31; ILN; Interleukin; interleukin 31; interleukin-31
• Common Name: IL-31
• Biological Activity: Determined by its ability to induce STAT3 activation in U87 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
• Content And Storage: -20°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Inflammation, Immunology
• Product Line: Gibco™
• Purity or Quality Grade: >98% by SDS-PAGE