Invitrogen™ Human IL-32 alpha ELISA Kit

Catalog No.	Quantity
EH274RBX5	5 x 96 Tests
EH274RBX10	10 x 96 Tests
EH274RB	96 Tests

Catalog No. EH274RBX5 Supplier Invitrogen™ Supplier No. EH274RBX5

Description

ELISA

Human IL-32 alpha quantitates human IL-32 alpha in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-32 alpha.

Interleukin 32 alpha (IL-32a) is a distinct isoform of the IL-32 cytokine, initially characterized as a transcript from activated NK cells. The gene encoding IL-32 is structured with eight exons, producing multiple alternatively spliced mRNA isoforms, including IL-32a. This isoform maintains similarity to other IL-32 variants, possessing sites for tyrosine sulfation, N-myristoylation, protein kinase C, casein kinase II phosphorylation, and RGD cell attachment, which are crucial for its functional interactions. IL-32a lacks a transmembrane region and is secreted, implicating a role in inflammation and immune processes by provoking pro-inflammatory cytokines while retaining certain anti-inflammatory features intracellularly. Particularly in the context of immune response, IL-32a is involved in defense mechanisms against intracellular pathogens and may have implications in cancerous processes, given its ability to influence cellular metabolism and proliferation. Its involvement in inflammation and immune regulation underlines its complex role in health and disease.

Specifications

• Assay Range: 4.1-1000 pg/mL
• Assay Sensitivity: 4.1 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: IL32; IL-32; IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin 32; interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cell transcript 4; natural killer cells protein 4; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; Tumor necrosis factor alpha-inducing factorIL32; IL-32; IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin 32; interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cell transcript 4; natural killer cells protein 4; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; Tumor necrosis factor alpha-inducing factor;
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL-32 alpha
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min