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Invitrogen™ eBioscience™ Human IL-33 Platinum ELISA Kit Non-distribution product as customer accommodation.

For the quantitative detection of human IL-33.

Manufacturer:  Invitrogen™ BMS2048

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Catalog No. 50-112-8949


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Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal polyclonal antibody to human IL-33
Biotin-Conjugate anti-human IL-33 polyclonal polyclonal antibody
Streptavidin-HRP
Human IL-33 Standard lyophilized, 500pg/mL upon reconstitution
Sample Diluent
Conjugate Diluent
Calibrator Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
Specifications

Specifications

O95760
Biotin
90865
Adhesive Plate Covers
96 tests
0.2 pg/mL
50μL
Human
1 hr 20 min
Human
Interleukin 33, DVS27-related protein, IL1F11
4.7%
7.8-500 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
4 hr 30 min
IL33
6.9%
HRP
Documents
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