Gibco™ Human IL-36 gamma (IL-1F9), Animal-Free Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human IL-36gamma is a 17.0 kDa protein containing 152 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.

Specifications

• Accession Number: Q9NZH8
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 56300
• Molecular Weight (g/mol): 17 kDa
• Name: Human IL-36 gamma (IL-1F9), Animal-Free
• Purification Method: HPLC, SDS-PAGE
• Quantity: 2 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: IL-1 epsilon; IL-1 related protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-related protein 2; IL1RP2; IL-1 RP2; IL36G; Interleukin; interleukin 1 family, member 9; interleukin 1-related protein 2; interleukin 36, gamma; interleukin-1 epsilon; Interleukin-1 family member 9; interleukin-1 homolog 1; Interleukin-36; Interleukin-36 gamma; UNQ2456/PRO5737
• Common Name: IL1F9
• Gene Symbol: IL36G
• Biological Activity: Measured by its ability to induce IL-8 production by human PBMCs.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND
• Content And Storage: -20°C
• Activity: Measured by its ability to induce IL-8 production by human PBMCs.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.