Gibco™ Human IL-5 Recombinant Protein

Catalog No.	Quantity
PHC0055	10 μg

Catalog No. PHC0055 Supplier Gibco™ Supplier No. PHC0055

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute recombinant human IL-5 in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to 280°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

Specifications

• Accession Number: P05113
• For Use With (Application): Bioactivity
• Formulation: 20mM sodium phosphate with no preservative; pH 7.5
• Gene ID (Entrez): 3567
• Molecular Weight (g/mol): 26 kDa
• Name: Human IL-5
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF
• Common Name: IL-5
• Gene Symbol: Il5
• Biological Activity: ED50 ≤ 0.15 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Disulfide-linked homodimer with each subunit containing 116 amino acid residues
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: ED50 ≤ 0.15 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Respiratory Research, Immunology
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC

Safety and Handling

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