Invitrogen™ Human IL-6 Recombinant Protein

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

Specifications

• Accession Number: P05231
• For Use With (Application): ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 3569
• Molecular Weight (g/mol): 20.95 kDa
• Name: Human IL-6
• Quantity: 50 μg
• Source: E. coli
• Regulatory Status: RUO
• Gene Alias: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
• Common Name: IL-6
• Gene Symbol: Il6
• Biological Activity: 1. Immobilized Human IL-6 Recombinant Protein (at 2 μg/mL (100 μL/well) can bind Human IL-6 R hFc, the EC50 of Human IL-6 R hFc is 3.0-10.0 ng/mL 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL 3.Loaded Recombinant Human IL-6 R Protein, His Tag on His1K Biosensor, can bind Human IL-6 Recombinant Protein with an affinity constant of 0.165 μM as determined in BLI assay (Sartorius Octet RED384)(Routinely tested).)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Human IL-6, amino acids Val30-Met212 (Accession # NP_000591.1) with an N-terminal Met
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: >95% by SDS-PAGE

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov