Invitrogen™ Human Importin alpha 2/KPNA2/SRP1-alpha ELISA Kit

Description

ELISA

Human Importin alpha 2/KPNA2/SRP1-alpha quantitates human Importin alpha 2/KPNA2/SRP1-alpha in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Importin alpha 2/KPNA2/SRP1-alpha.

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1, which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in VJ recombination.

Specifications

• Accession Number: 2410044B12Rik; IMA2; importin; importin alpha 2; Importin alpha P1; Importin subunit alpha-1; importin subunit alpha-2; importin-alpha-P1; IPOA 1; IPOA1; IPOA1 QIP2; karyopherin (importin) alpha 2; Karyopherin A2; karyopherin alpha 2; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); karyopherin subunit alpha 2; karyopherin subunit alpha-2; Kpna2; m-importin-alpha-P1; nuclear import protein; pendulin; Pore targeting complex 58 kDa subunit; PTAC58; QIP2; RAG cohort 1; RAG cohort protein 1; RCH 1; RCH1; SRP 1; SRP1; SRP1 alpha; SRP1alpha; SRP1-alpha
• Assay Range: 2 to 500 ng/mL
• Assay Sensitivity: 2 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3838
• Gene Symbol: KPNA2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: KPNA2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins