Human INS Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of INS in serum plasma cell culture supernates and other biological fluids It provides a detection range of 0 16-10 ng/mL with a sensitivity of 0 053 ng/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human INS Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human INS Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human INS biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color