Invitrogen™ Human IL-1 alpha Recombinant Protein

Catalog No.	Quantity
ENRIL1AI	20 μg

Catalog No. ENRIL1AI Supplier Invitrogen™ Supplier No. RIL1AI

Description

Recombinant Protein

Mature human IL-1a was expressed in Pichia pastoris, KM71H. GenBank Accession # P01583, amino acids 113 - 271. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: SAPFSFLSNV. Purity is >95% by SDS-PAGE analysis. Activity: The ED50 typically falls into the range of 7.9 - 13.5 pg/mL in proliferation assays of mouse D10S cells. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Specifications

• Accession Number: P01583
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3552
• Molecular Weight (g/mol): 18 kDa
• Name: Human IL-1 alpha
• Quantity: 20 μg
• Source: Yeast
• Regulatory Status: RUO
• Gene Alias: CTLA8; hematopoietin-1; IL 1 A; IL 1 A; IL1; IL-1 alpha; IL17; IL1A; Il-1 A; IL-1 alpha; IL1-ALPHA; IL1F1; IL1a; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1 A; interleukin 1-alpha; interleukin 1-alpha (AA 1 - 152); Interleukin1 alpha; interleukin-1 alpha; interleukin-1 alpha precursor; Interleukin-17 A; preinterleukin 1 alpha; pre-interleukin-1 alpha; pro-interleukin-1-alpha; RP23-160G19.8
• Common Name: IL-1 alpha
• Gene Symbol: IL1A
• Biological Activity: ED50 = 7.9 - 13.5 pg/mL; determined by the dose-dependent proliferation of mouse D10S cells.
• Conjugate: Unconjugated
• Species: Yeast
• Recombinant: Recombinant
• Sequence: Human IL-1 alpha, amino acids 113-271 (Accession # P01583)
• Content And Storage: 4°C
• Expression System: P. pastoris
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95% by SDS-PAGE