Invitrogen™ Human IL-10 Recombinant Protein

Description

Recombinant Protein

Use: IL-10 exerts activity in the range of 0.2 to 20 ng/mL for most in vitro applications. Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 μg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V). Lyophilized product is stable until expiration if stored desiccated below 0°C. Reconstituted undiluted cytokine should be stored in working aliquots at -70°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Accession Number: P22301
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 3586
• Molecular Weight (g/mol): 18.6 kDa
• Name: Human IL-10
• pH Range: 7.2
• Quantity: 25 μg
• Source: E. Coli
• Regulatory Status: RUO
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
• Common Name: IL-10
• Gene Symbol: IL10
• Biological Activity: ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of human IL-4 co-stimulated MC/9 cells.
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Human IL-10 recombinant protein contains 161 amino acids
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >98% by SDS-PAGE and FPLC