Invitrogen™ Human IL-4 Recombinant Protein

Description

Recombinant Protein

Mature human IL-4 was expressed in Pichia pastoris, X-33. GenBank Accession # P05112, amino acids 25 - 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLQEI. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant human IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Accession Number: P05112
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3565
• Molecular Weight (g/mol): 15 kDa
• Name: Human IL-4
• Quantity: 20 μg
• Source: Yeast
• Regulatory Status: RUO
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4 delta2; interleukin 4 variant IL-4 delta3; interleukin 4 variant IL-4 int2A; interleukin 4 variant IL-4 int3A; interleukin 4 variant IL-4 int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Common Name: IL-4
• Gene Symbol: IL4
• Biological Activity: Determined by the proliferation of human TF-1 cells.
• Conjugate: Unconjugated
• Species: Yeast
• Recombinant: Recombinant
• Sequence: Human IL-4, amino acids 25-153 (Accession # P05112)
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: P. pastoris
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95% by SDS-PAGE