Invitrogen™ Human IL-6 Recombinant Protein

Description

Recombinant Protein

Mature human IL-6 was expressed in Pichia pastoris, X-33. GenBank Accession # P05231, amino acids 30 - 212. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VPPGEDSKDV. Purity is >95% by SDS-PAGE analyses. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

Specifications

• Accession Number: P05231
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3569
• Molecular Weight (g/mol): 20.8 kDa
• Name: Human IL-6
• Quantity: 20 μg
• Source: Yeast
• Regulatory Status: RUO
• Gene Alias: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
• Common Name: IL-6
• Gene Symbol: IL6
• Conjugate: Unconjugated
• Species: Yeast
• Recombinant: Recombinant
• Sequence: Human IL-6, amino acids 30-212 (Accession # P05231)
• Content And Storage: 4°C
• Expression System: P. pastoris
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95% by SDS-PAGE

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov