Invitrogen™ Human IL-7 Recombinant Protein

Description

Recombinant Protein

Use: Human IL-7 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications. Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 μg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V). Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

Specifications

• Accession Number: P13232, P16871
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 3574, 3575
• Molecular Weight (g/mol): 17.4 kDa
• Name: Human IL-7
• Quantity: 5 μg
• Source: E. Coli
• Regulatory Status: RUO
• Gene Alias: A630026I06Rik; H-IL-7; hlb368; IL7; Il-7; ILN; Interleukin; interleukin 7; Interleukin7; interleukin-7; M-IL-7
• Common Name: IL-7
• Gene Symbol: IL7
• Biological Activity: ED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of mouse 2E8 cells.
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Human IL-7 recombinant protein contains 153 amino acids
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >98% by SDS-PAGE and HPLC