Invitrogen™ Human IP-10/CXCL10 Instant ELISA™ Kit

Catalog No.	Quantity
50-182-34	128 Tests

Catalog No. 50-182-34 Supplier Invitrogen™ Supplier No. BMS284INST

Description

ELISA

The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu sIP-10/CXCL10 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, synovial fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIP-10/CXCL10. Principle of the method The Human sIP-10/CXCL10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

Specifications

• Accession Number: P02778
• Assay Range: 3.1 to 200 pg/mL
• Assay Sensitivity: 1.0 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Synovial Fluid, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3627
• Gene Symbol: C7,IFI10,INP10,IP-10,SCYB10,crg-2,gIP-10,mob-1
• Interassay CV: 10.4%
• Intraassay CV: 6.7
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Synovial Fluid 10 μL, Urine 10 μL
• Storage Requirements: -20°C
• Synonym: Interferon-gamma-inducible protein 10, CXCL10
• Target: CXCL10 (IP-10)
• Test Time: 15 mins
• Total Assay Time: 3 hrs 10 mins