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Invitrogen™ IP-10/CXCL10 Human Instant ELISA™ Kit Non-distribution product as customer accommodation.

Instant ELISA Kit

Manufacturer:  Invitrogen™ BMS284INST

Catalog No. 50-182-34


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Includes: Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human IP-10, Biotin-Conjugate (anti-IP-10 monoclonal antibody), Sample Diluent, and Streptavidin-HRP, lyophilized
Aluminium pouch(es) with a human IP-10 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers

Description

Description

The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu sIP-10/CXCL10 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, synovial fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIP-10/CXCL10. Principle of the method The Human sIP-10/CXCL10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL10 ( C-X-C Motif Chemokine Ligand 10) is a 10 kDa protein and member of the CXC family that acts by binding to CXCR3. Binding of CXCL10 to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. CXCL10 is a pro-inflammatory chemoattractant cytokine that attracts activated T lymphocytes. CXCL10 is constitutively expressed at low levels in lymph organs, and the expression is highly induced by IFN- gamma and to some extent by IL12. It is an important biomarker for diagnosis of many clinical conditions, and it plays an important role in host defense and inflammation. CXCL10 exhibits an angiostatic and an antitumor role that is also involved in Th1 inflammatory diseases, Hashimoto's thyroiditis, Graves ft. disease and Type 1 diabetes mellitus.
Specifications

Specifications

P02778
Biotin
3627
Adhesive Plate Covers
128 tests
1.0 pg/mL
10μL
Human
20 min
Human
Interferon-gamma-inducible protein 10, CXCL10
6.7%
3.1-200 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Amniotic Fluid, Plasma, Serum, Supernatants, Synovial Fluid, Urine
Amniotic Fluid (10μL), Plasma (10μL), Serum (10μL), Supernatants (10μL), Synovial Fluid (10μL), Urine (10μL)
−20°C
3 hr 10 min
CXCL10
10.4%
HRP
Documents
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