Invitrogen™ Human JAM-B ELISA Kit

Catalog No.
EH284RBX10	10 x 96 Tests; HRP

Catalog No. EH284RBX10 Supplier Invitrogen™ Supplier No. EH284RBX10

Description

ELISA

Human JAM-B quantitates human JAM-B in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human JAM-B.

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.

Specifications

• Accession Number: P57087
• Assay Range: 0.068-50 ng/mL
• Assay Sensitivity: 0.068 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110002N23Rik; 2410030G21Rik; 2410167M24Rik; AU016127; C21orf43; CD322; Jam2; JAM-2; JAMB; JAM-B; JAM-IT/VE-JAM; Jcam2; junction adhesion molecule 2; junction adhesion molecule B; junction cell adhesion molecule 2; junctional adhesion molecule 2; Junctional adhesion molecule B; PRO245; RP23-253E17.2; UNQ219/PRO245; Vascular endothelial junction-associated molecule; VEJAM; VE-JAM1110002N23Rik; 2410030G21Rik; 2410167M24Rik; AU016127; C21orf43; CD322; Jam2; JAM-2; JAMB; JAM-B; JAM-IT/VE-JAM; Jcam2; junction adhesion molecule 2; junction adhesion molecule B; junction cell adhesion molecule 2; junctional adhesion molecule 2; Junctional adhesion molecule B; PRO245; RP23-253E17.2; UNQ219/PRO245; Vascular endothelial junction-associated molecule; VEJAM; VE-JAM;
• Gene ID (Entrez): 58494
• Gene Symbol: JAM2
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: JAM2
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min