Invitrogen™ Human KISS1 ELISA Kit

Catalog No.	Quantity
EEL080	96 Tests

Catalog No. EEL080 Supplier Invitrogen™ Supplier No. EEL080

Description

ELISA

The Human Kisspeptin 1 (KISS1) ELISA quantitates KISS1 in serum, plasma, and other biological fluids The Human Kisspeptin 1 (KISS1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH neurons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues.

Specifications

• Accession Number: Q15726
• Assay Range: 31.25-2000 pg/mL
• Assay Sensitivity: 18.75 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: KISS1
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 0610012C01Rik; Eseptin; HH13; KISS1; KiSS-1; KiSS-1 metastasis-suppressor; Kiss1 metastasis-suppressor; Kiss1 variant E1a-E2b-E3; Kiss1 variant E1b-E2a-E3; Kiss1 variant E1b-E2b-E3; kisspeptin; Kisspeptin-1; Kisspeptin-10; Kisspeptin-13; Kisspeptin-14; Kisspeptin-52; Kisspeptin-54; malignant melanoma metastasis-suppressor; metastasis-suppressor KiSS-1; metastatin; metastin; Metastin45-54; MLL septin-like fusion; PP5098; prepro-kisspeptin0610012C01Rik; Eseptin; HH13; KISS1; KiSS-1; KiSS-1 metastasis-suppressor; Kiss1 metastasis-suppressor; Kiss1 variant E1a-E2b-E3; Kiss1 variant E1b-E2a-E3; Kiss1 variant E1b-E2b-E3; kisspeptin; Kisspeptin-1; Kisspeptin-10; Kisspeptin-13; Kisspeptin-14; Kisspeptin-52; Kisspeptin-54; malignant melanoma metastasis-suppressor; metastasis-suppressor KiSS-1; metastatin; metastin; Metastin45-54; MLL septin-like fusion; PP5098; prepro-kisspeptin;
• Gene ID (Entrez): 3814
• Gene Symbol: KISS1
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: KISS1
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min