Invitrogen™ Human LAG-3 (CD223) Fc Chimera Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 1.25 mg/mL. Concentration is measured by UV.

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

Specifications

• Accession Number: P18627
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: 0.1 M Glutamine, 20 mM sodium phosphate with 0.01% mannitol, 0.01% Tween 20, 0.01% Tween 80, 0.1 M arginine, 10% glycerol, 150 mM NaCl, 5-8% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 3902
• Molecular Weight (g/mol): 84.19 kDa
• Name: Human LAG-3 (CD223) Fc Chimera
• Purification Method: SDS-PAGE
• Quantity: 500 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3 lymphocyte activating 3; soluble LAG3
• Common Name: LAG-3 (CD223)
• Gene Symbol: LAG3
• Biological Activity: Immobilized LAG3 Protein, Human, Recombinant (ECD, hFc Tag) (at 2 μg/mL (100 μL/well) can bind Anti-LAG3 (BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human LAG3, amino acids Met1-Leu450 (Accession # NP_002277.4) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized LAG3 Protein, Human, Recombinant (ECD, hFc Tag) (at 2 μg/mL (100 μL/well) can bind Anti-LAG3 (BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.