Invitrogen™ Human LAG-3 (CD223) Recombinant Protein, Biotin

Catalog No.	Quantity
PIRP88098	100 μg

Catalog No. PIRP88098 Supplier Invitrogen™ Supplier No. RP88098

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

LAG-3 is a 70-kDa surface glycoprotein in the immunoglobulin superfamily, with structural homology to CD4. It binds to MHC class II molecules with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on the surface of activated T cells, including regulatory T cells, and NK cells. Notably, CD8+ T cells express LAG-3 at significantly higher levels than CD4+ T cells. Additionally, the coexpression of LAG-3 and CD49b has been proposed as a marker for identifying Type 1 regulatory T cells (Tr1 cells) in both humans and mice. This highlights LAG-3s role in immune regulation and its potential as a target for therapeutic interventions in immune-related conditions.

Specifications

• Accession Number: P18627
• Concentration: 0.25 mg/mL
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 3902
• Molecular Weight (g/mol): 47.0 kDa
• Name: Human LAG-3 (CD223)
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3lymphocyte activating 3; soluble LAG3
• Common Name: LAG-3 (CD223)
• Gene Symbol: LAG3
• Biological Activity: Immobilized Human LAG3, Biotinylated (at 2 μg/mL (100 μL/well), EC50 is 5-15 ng/mL Can bind Human FGL1 (hFc Tag), the EC50 is 20-65 ng/mL)
• Product Type: Protein
• Conjugate: Biotin
• Recombinant: Recombinant
• Sequence: Human LAG3, amino acids Met1-Arg440 (Accession # NP_002277.4)
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized Human LAG3, Biotinylated at 2 μg/mL (100 μL/well), EC50 is 5-15 ng/mL. Can bind Human FGL1 (hFc Tag), the EC50 is 20-65 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.