Invitrogen™ Human Laminin alpha 4 ELISA Kit

Description

ELISA

Human Laminin alpha 4 quantitates human Laminin alpha 4 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Laminin alpha 4.

Laminin is a non-collagenous protein of basement membranes. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400 kD) and three short arms (200 kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro.

Specifications

• Accession Number: CMD1JJ; LAMA3; LAMA4; LAMA4*-1; laminin [a]4; laminin alpha 4 chain; laminin subunit alpha 4; laminin subunit alpha-4; laminin, alpha 4; laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha; RGD1560062
• Assay Range: 1.63 to 400 ng/mL
• Assay Sensitivity: 1.63 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3910
• Gene Symbol: LAMA4
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Laminin alpha-4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov