Invitrogen™ Human Lefty-A ELISA Kit

Description

ELISA

Human Lefty-A quantitates human Lefty-A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Lefty-A.

Left-right determination factor 1 and 2 (Lefty1/2) belong to the TGF-beta superfamily of proteins and are responsible for determination of left-right positioning of visceral organs during embryogenesis. Lefty1 acts as a regulator of Lefty2 and NODAL as the lack of the former resulted in bilateral expression of nodal, lefty2 and Pitx2 (a homeobox gene normally expressed on the left side and blocks the signal for leftness). Lefty2 may play a role in endometrial bleeding.

Specifications

• Accession Number: 6030463A22Rik; AV214969; EBAF; Ebaf2; endometrial bleeding associated factor; endometrial bleeding-associated factor; LEFTA; Leftb; Left-right determination factor 2; left-right determination factor A; Left-right determination factor B; left-right determination, factor A; Lefty2; LEFTYA; MGC46222; Protein lefty-2; protein lefty-A; Protein lefty-B; PSEC0024; TGFB4; TGF-beta-4; Transforming growth factor beta-4
• Assay Range: 0.82 to 200 ng/mL
• Assay Sensitivity: 0.82 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7044
• Gene Symbol: LEFTY2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: LEFTY2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov