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Invitrogen™ Human Lefty-A ELISA Kit

Catalog No. EH298RBX5
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EH298RB 96 Tests
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ELISA

Human Lefty-A quantitates human Lefty-A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Lefty-A.

Left-right determination factor 1 and 2 (Lefty1/2) belong to the TGF-beta superfamily of proteins and are responsible for determination of left-right positioning of visceral organs during embryogenesis. Lefty1 acts as a regulator of Lefty2 and NODAL as the lack of the former resulted in bilateral expression of nodal, lefty2 and Pitx2 (a homeobox gene normally expressed on the left side and blocks the signal for leftness). Lefty2 may play a role in endometrial bleeding.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O00292
Assay Range 0.82-200 ng/mL
Assay Sensitivity 0.82 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 6030463A22Rik; AV214969; Ebaf; Ebaf2; endometrial bleeding associated factor; endometrial bleeding-associated factor; LEFTA; Leftb; Left-right determination factor 2; left-right determination factor A; Left-right determination factor B; left-right determination, factor A; LEFTY2; LEFTYA; MGC46222; protein lefty-2; Protein lefty-A; protein lefty-B; PSEC0024; TGFB4; TGF-beta-4; Transforming growth factor beta-46030463A22Rik; AV214969; Ebaf; Ebaf2; endometrial bleeding associated factor; endometrial bleeding-associated factor; LEFTA; Leftb; Left-right determination factor 2; left-right determination factor A; Left-right determination factor B; left-right determination, factor A; LEFTY2; LEFTYA; MGC46222; protein lefty-2; Protein lefty-A; protein lefty-B; PSEC0024; TGFB4; TGF-beta-4; Transforming growth factor beta-4;
Gene ID (Entrez) 7044
Gene Symbol LEFTY2
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target LEFTY2
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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