Abnova™ Human lg-kappa CISH Probe

Human lg-kappa CISH Probe is designed for the qualitative detection of human Ig-kappa light chain mRNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0030

Catalog No. 89-335-581

Description

The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

A positive reactivity in plasma B-cells is indicated by cytoplasmic staining. In lymphoid tissue, the normal kappa-to-lambda ratio is roughly 2:1, an indication for monoclonality is given if the kappa-to-lambda ratio is greater than 3:1 or less than 0.3:1. Visualization of signals should be performed at least at 100-fold magnification resulting in easily visible signals. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei, and nuclei with weak signal intensity.

Specifications

• For Use With (Application): CISH-P
• Includes: Reagent Provided:
  
  Digoxigenin-labeled oligonucleotides targeting mRNA sequences encoding Ig-kappa light chain constant regions.
• Content And Storage: Ready-to-use. No reconstitution, mixing, or dilution required. Bring to room temperature and mix briefly before use. Typically used with Human Ig-lambda CISH Probe