Invitrogen™ Human Livin ELISA Kit

Catalog No.	Quantity
EH302RBX5	5 x 96 Tests
EH302RBX10	10 x 96 Tests
EH302RB	96 Tests

Catalog No. EH302RBX5 Supplier Invitrogen™ Supplier No. EH302RBX5

Description

ELISA

Human Livin quantitates human Livin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Livin.

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, it has been identified that a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells.

Specifications

• Accession Number: Q96CA5
• Assay Range: 0.1-25 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; baculoviral IAP repeat-containing 7 (livin); baculoviral IAP repeat-containing protein 7; Baculoviral IAP repeat-containing protein 7 30 kDa subunit; Baculoviral IAP repeat-containing protein 7 30kDa subunit; Birc7; E130019N06; KIAP; Kidney inhibitor of apoptosis protein; LIVIN; livin inhibitor of apoptosis; melanoma inhibitor of apoptosis protein; MLIAP; ML-IAP; RGD1562883; RING finger protein 50; RING-type E3 ubiquitin transferase BIRC7; RNF50; RP11-261N11.7; Truncated livin; UNQ5800/PRO19607/PRO21344baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; baculoviral IAP repeat-containing 7 (livin); baculoviral IAP repeat-containing protein 7; Baculoviral IAP repeat-containing protein 7 30 kDa subunit; Baculoviral IAP repeat-containing protein 7 30kDa subunit; Birc7; E130019N06; KIAP; Kidney inhibitor of apoptosis protein; LIVIN; livin inhibitor of apoptosis; melanoma inhibitor of apoptosis protein; MLIAP; ML-IAP; RGD1562883; RING finger protein 50; RING-type E3 ubiquitin transferase BIRC7; RNF50; RP11-261N11.7; Truncated livin; UNQ5800/PRO19607/PRO21344;
• Gene ID (Entrez): 79444
• Gene Symbol: BIRC7
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Livin
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min