Invitrogen™ Human LP-a ELISA Kit

Catalog No.	Quantity
EEL166	96 Tests

Catalog No. EEL166 Supplier Invitrogen™ Supplier No. EEL166

Description

ELISA

The Human Apolipoprotein a (LP a) ELISA quantitates LP a in serum, plasma and other biological fluids. Principle of the method The Human Apolipoprotein a (LP a) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

The protein encoded by this gene is a serine proteinase that inhibits the activity of tissue-type plasminogen activator I. The encoded protein constitutes a substantial portion of lipoprotein(a) and is proteolytically cleaved, resulting in fragments that attach to atherosclerotic lesions and promote thrombogenesis. Elevated plasma levels of this protein are linked to atherosclerosis. Depending on the individual, the encoded protein contains 2-43 copies of kringle-type domains. The allele represented here contains 15 copies of the kringle-type repeats and corresponds to that found in the reference genome sequence.

Specifications

• Accession Number: P08519
• Assay Range: 0.23-15 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AK38; antiangiogenic AK38 protein; apo(a); APOA; apolipoprotein(a); lipoprotein(a); lipoprotein, Lp(a); LP; lp(a); LPAAK38; antiangiogenic AK38 protein; apo(a); APOA; apolipoprotein(a); lipoprotein(a); lipoprotein, Lp(a); LP; lp(a); LPA;
• Gene ID (Entrez): 4018
• Gene Symbol: LPA
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: LPA
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min