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Invitrogen™ Human Lp-PLA2/PLA2G7/PAF-AH ELISA Kit

Catalog No. EH304RBX10
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EH304RBX10 10 x 96 Tests
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Catalog No. EH304RBX10 Supplier Invitrogen™ Supplier No. EH304RBX10
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ELISA

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of thesn2 ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL-associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q13093
Assay Range 8.19 to 2,000 ng/mL
Assay Sensitivity 8.1 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7941
Gene Symbol PLA2G7
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target PLA2G7
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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