Gibco™ Human M-CSF, AOF Recombinant Protein

Catalog No.	Quantity
PHG6054	10 μg

Catalog No. PHG6054 Supplier Gibco™ Supplier No. PHG6054

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute M-CSF in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein. Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

Specifications

• Accession Number: P09603
• For Use With (Application): Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 1435
• Molecular Weight (g/mol): 36.8 kDa
• Name: Human M-CSF, AOF
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/mL
• Gene Alias: C87615; colony stimulating factor 1; colony stimulating factor 1 (macrophage); colony-stimulating factor-1 splice variant; CSF1; CSF-1; Csfm; H-MCSF; lanimostim; macrophage colony stimulating factor 1; macrophage colony stimulating factor alpha; macrophage colony stimulating factor beta; macrophage colony-stimulating factor; macrophage colony-stimulating factor 1; macrophage colony-stimulating factor beta; macrophage-colony stimulating factor alpha; MCSF; MCSF alpha; MCSFBETA; MGC31930; M-MCSF; op; osteopetrosis; Processed macrophage colony-stimulating factor 1
• Common Name: M-CSF
• Gene Symbol: Csf1
• Biological Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of mouse M-NFS-60 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of mouse M-NFS-60 cells.
• Bioassay: proliferation of murine M-NFS-60 cells
• Endotoxin Level: <1 EU/ml
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: GM-CSF, G-CSF, & M-CSF
• Research Category: Cardiovascular Research, Stem Cell Research, Inflammation
• Product Line: Gibco
• Purity or Quality Grade: >98% by SDS-PAGE

Safety and Handling

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