Invitrogen™ Human MCP-1 ELISA Kit

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MCP-1
HRP-Conjugate anti-human MCP-1 monoclonal antibody
Human MCP-1 Standard lyophilized, 2ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

ELISA

The Human Monocyte Chemoattractant Protein-1(Hu MCP-1) ELISA quantitates Hu MCP-1 in human serum, plasma,amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MCP-1. Principle of the method The Human MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Specifications

• Accession Number: P13500
• Assay Range: 15.6 to 1,000 pg/mL
• Assay Sensitivity: 2.3 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6347
• Gene Symbol: GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF
• Interassay CV: 8.7%
• Intraassay CV: 4.7
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 20 μL, Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Monocyte chemoattractant protein 1, MCAF, HC11
• Target: CCL2 (MCP-1)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 10 mins