Invitrogen™ Human MEPE/OF45 ELISA Kit

Catalog No.	Quantity
EH320RBX5	5 x 96 Tests
EH320RB	96 Tests

Catalog No. EH320RBX5 Supplier Invitrogen™ Supplier No. EH320RBX5

Description

ELISA

Human MEPE/OF45 quantitates human MEPE/OF45 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MEPE/OF45.

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. MEPE promotes renal phosphate excretion and modulates mineralization. It can be phosphorylated by secreted protein kinases. MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines. It is a 525-amino acid protein with a calculated molecular weight of 58 kDa.

Specifications

• Accession Number: matrix extracellular phosphoglycoprotein; matrix extracellular phosphoglycoprotein with ASARM motif (bone); matrix, extracellular phosphoglycoprotein with ASARM motif (bone); Mepe; OF45; Osteoblast/osteocyte factor 45; Osteoregulin
• Assay Range: 26 to 2,000 ng/mL
• Assay Sensitivity: 8.4 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 56955
• Gene Symbol: MEPE
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: MEPE
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov