Invitrogen™ Human MFG-E8 ELISA Kit

Description

ELISA

Human MFG-E8 quantitates human MFG-E8 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MFG-E8.

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants.

Specifications

• Accession Number: AA408458; Ags; AI325141; BA46; Breast epithelial antigen BA46; EDIL1; EGF/factor VIII; HMFG; hP47; HsT19888; lactadherin; Lactadherin short form; Medin; Mfge8; MFG-E8; MFGM; MFGME8; MFGMP-E8; milk fat globule glycoprotein; milk fat globule membrane; milk fat globule membrane protein; Milk fat globule-EGF factor 8; milk fat globule-EGF factor 8 protein; MP47; O-acetyl disialoganglioside synthase; O-acetyl GD3 ganglioside synthase; O-acetyltransferase milk fat globule membrane protein; O-acetyltransferase Milk fat glolbule membrane protein; OAcGD3S; P47; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47; Sperm surface protein SP47
• Assay Range: 0.1 to 25 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4240
• Gene Symbol: MFGE8
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: MFGE8 (Lactadherin)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins