Gibco™ Human MICA Fc Recombinant Protein, PeproTech®

Catalog No.	Quantity
50-003-31934	100 μg
31-052-10UG	10 μg
31-052-1MG	1 mg
50-003-31922	250 μg
50-003-31920	500 μg
31-052-50UG	50 μg

Catalog No. 50-003-31934 Supplier Gibco™ Supplier No. 31052100UG

Description

Recombinant Protein

Recombinant Human MICA Fc is an 117.6 kDa glycosylated, disulfide-linked homodimer whose 518 amino acid monomer consists of the 285 amino acid N-terminal portion of the extracellular domain fused to the 231-amino-acid residue C-terminal Fc region og human IgG by two glycine residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis.

Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

Specifications

• Accession Number: Q29983
• For Use With (Application): Binding Assay, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 100507436
• Molecular Weight (g/mol): 58.9 kDa
• Name: Human MICA Fc
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: DAMA-345G11.2; HLA class I antigen; MHC class I chain-related protein A; MHC class I polypeptide-related sequence A; MHC class I related sequence A; MICA; MIC-A; PERB11.1; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A
• Common Name: MICA
• Gene Symbol: MICA
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: EPHSLRYNLT VLSWDGSVQS GFLTEVHLDG QPFLRCDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETKEWTMP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLKSGVVLRR TVPPMVNVTR SEASEGNITV TCRASGFYPW NITLSWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC QGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHWQGGPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
• Activity: Determined by its ability to bind immobilized human NKG2D Fc Chimera in a functional ELISA.
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.