Invitrogen™ Human MIS/AMH Rapid ELISA Kit

Catalog No.	Quantity
EELR022	96 Tests

Catalog No. EELR022 Supplier Invitrogen™ Supplier No. EELR022

Description

ELISA

The Human Anti Mullerian Hormone (AMH) ELISA quantitates AMH in serum and plasma. Principle of the method The Human AMH solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140 kDa disulfide linked precursor that is cleaved to release the mature 30 kDa homodimer. Originally classified as a fetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.

Specifications

• Accession Number: P03971
• Assay Range: 78.13-5000 pg/mL
• Assay Sensitivity: 46.88 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AMH; Anti - Mullerian hormone (Mulerian inhibiting substance); anti-Muellerian hormone; anti-Mullerian hormone; MIF; MIS; Muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian inhibiting factor; Mullerian inhibiting substanceAMH; Anti - Mullerian hormone (Mulerian inhibiting substance); anti-Muellerian hormone; anti-Mullerian hormone; MIF; MIS; Muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian inhibiting factor; Mullerian inhibiting substance;
• Gene ID (Entrez): 268
• Gene Symbol: AMH
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: AMH
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min