Invitrogen™ Human MLNR (aa 317-338) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP109772	100 μL

Catalog No. RP109772 Supplier Invitrogen™ Supplier No. RP109772

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (36%), Rat (36%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-144985. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Motilin is a widely conserved 22 amino acid peptide hormone secreted from enterochromaffin cells of the gastrointestinal tract. Within the intestines, motilin potentiates intestinal motility by inducing contractions of the duodenum and through binding to surface receptors, designated GPR38 (G-protein coupled receptor 38). These motilin receptors are predominantly expressed in stomach, thyroid and bone marrow, and they are related to other G-protein coupled receptors located in the pituitary and hypothalamus, which mediate the signaling for growth hormone secretagogues. The gene encoding GPR38 is alternately spliced at the carboxy terminus to generate two related proteins that are designated GPR38-A and GPR38-B. Sequence comparisons of the two isoforms indicate that GPR38-A contains seven transmembrane domains while GPR38-B is predicted to contains only five transmembrane regions. Consistent with other G-protein coupled receptors, GPR38 activates phospholipase C signal transduction pathways and induces intracellular calcium mobilization after binding of motilin.

Specifications

• Accession Number: O43193
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 2862
• Name: Human MLNR (aa 317-338) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: G protein-coupled receptor 38; GPR38; G-protein coupled receptor 38; MLNR; Motilin receptor; MTLR; MTLR1; thyrotropin releasing hormone receptor 2; Trhr2
• Common Name: MLNR
• Gene Symbol: MLNR
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: GRIIYINTEDSRMMYFSQYFNI
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining